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Image Search Results
Journal: Frontiers in Oncology
Article Title: RhoC Modulates Cell Junctions and Type I Interferon Response in Aggressive Breast Cancers
doi: 10.3389/fonc.2021.712041
Figure Lengend Snippet: RhoC expression influences gene and protein expression of Type I interferon signaling response. (A) Type I interferon signaling pathway and the genes significantly downregulated in crRhoC knockout cells compared to wild-type, as measured in RNAseq. Genes in blue circles were downregulated in crRhoC compared to wild-type; genes in grey circles were not significantly differentially expressed between crRhoC and wild-type. (B) Western blot of interferon signaling markers in MDA 231 wild-type and crRhoC cells—crRhoC cells have increased p-STAT2 and IRF9 compared to wild type. (C) Western blot of interferon signaling markers in SUM 149 wild-type and crRhoC cells—crRhoC cells have decreased p-STAT2 and STAT2 compared to wild type. (D) Short-term and long-term signaling through type I interferon signaling pathways. Short-term interferon signaling is driven by phosphorylated STAT1 and STAT2 complexed with IRF9 that translocates to the nucleus, binds to interferon stimulated response elements (ISREs), and promotes transcription of interferon stimulated genes (ISGs); phosphorylation of STAT1 and STAT2 peak about 2 hours after treatment with a type I interferon. Long-term signaling is driven by unphosphorylated STAT1 and STAT2 complexed with IRF9, and peaks around 72 hours after treatment with type I interferons. Genes listed in order of decreasing magnitude of log fold-change.
Article Snippet: The following primary antibodies were used: anti-E-cadherin rabbit polyclonal antibody (ThermoFisher #PA5-32178) at 1:500 dilution, anti-β-catenin mouse monoclonal antibody (Invitrogen #MA1-300) at 1:500 dilution, anti-ZO-1 mouse monoclonal antibody (Invitrogen #33-9100) at 1:150 dilution for immunofluorescent staining and 1:200 for Western Blot, anti-Occludin rabbit polyclonal antibody (Zymed #71-1500) at 1:300 dilution for immunofluorescent staining and Western Blot, anti-p-STAT1 rabbit monoclonal antibody (CST #9167) at 1:1000 dilution, anti-STAT1 rabbit monoclonal antibody (CST #9172) at 1:1000 dilution,
Techniques: Expressing, Knock-Out, Western Blot, Protein-Protein interactions, Phospho-proteomics